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Polymerase-endonuclease amplification reaction (PEAR) is a DNA amplification technology for the amplification of oligonucleotides. A target oligonucleotide and a tandem repeated antisense probe are subjected to repeated cycles of denaturing, annealing, elongation and cleaving, in which thermostable DNA polymerase elongation and strand slipping generate duplex tandem repeats, and thermostable endonuclease (PspGI) cleavage releases monomeric duplex oligonucleotides. PEAR has the potential to be a useful tool for: # Large-scale production of oligonucleotides. # PEAR is a minimal DNA replication system, so it can be considered as a minimal life system. it is of therectical interests to study the origin and evolution of repetitive DNA. # The repetitive DNA products can be transferred directly into cells or organisms to study the function of the repetitive DNA. ==References== 2. Biao Li, Shihua Dong, Jiajun Wu, Jianye Zhang, Gang Chen, Quanjiang Dong, Xinhong Zhu, Xiaolong Wang * (2013) Preparation of 5′-O-(1-Thiotriphosphate)-Modified Oligonucleotides Using Polymerase-Endonuclease Amplification Reaction (PEAR). PLoS ONE 8(7): e67558. doi:10.1371/journal.pone.0067558 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Polymerase-endonuclease amplification reaction」の詳細全文を読む スポンサード リンク
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